Figure 2
From: Andrographolide reduces cognitive impairment in young and mature AβPPswe/PS-1 mice
ANDRO reduces tau phosphorylation in the brains of AβPPswe/PS1 mice of different ages. (a) Immunoblot of hippocampus homogenates from young (7-month-old) AβPPswe/PS-1 mice treated with vehicle (control, white bars) or ANDRO (black bars) using the PHF-1 and AT8 antibodies. Graph corresponds to the densitometric analysis of bands normalized against β-tubulin (loading control) and compared with AβPPswe/PS-1 mice treated with vehicle or with ANDRO. (b) AT8-positive cells are detected in other regions in young (7-month-old) AβPPswe/PS-1 control or ANDRO-treated mice. Graphs show the quantification of the number of AT8-positive neurons per area in mm2 and near amyloid plaques, as detected by ThS staining. Positive neurons and amyloid plaques are indicated by black and white arrows, respectively. (c) Immunoblot of hippocampus homogenates from mature (12-month-old) AβPPswe/PS-1 control and ANDRO-treated mice using the PHF-1 and AT8 antibodies. Graph corresponds to the densitometric analysis of bands normalized against a loading control and compared with AβPPswe/PS-1 control and ANDRO-treated mice. (d) AT8-positive cells are detected in other regions in AβPPswe/PS-1 control and ANDRO-treated mice. The graphs show the quantification of the number of AT8-positive neurons per area in mm2 and near amyloid plaques, as detected by ThS staining. Positive neurons and amyloid plaques are indicated by black and white arrows, respectively. Analysis of AT8-positive neurons per plaque. Three animals were used per experimental group. Data are presented as mean ± SEM. Statistical differences were calculated by Student’s t test, followed by Dunnett’s post hoc test. Asterisks indicate statistically significant differences (*p < 0.05).