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Figure 3 | Molecular Neurodegeneration

Figure 3

From: Andrographolide reduces cognitive impairment in young and mature AβPPswe/PS-1 mice

Figure 3

ANDRO recovers synaptic proteins, increase levels of β-catenin and reduce levels of active of GSK-3β. (a) Immunoblots of total postsynaptic proteins (PSD-95, GluA2, Shank and GluN2B) and presynaptic proteins (SYP and VAMP) extracts from the hippocampi of 7-month-old AβPP/PS1 control and ANDRO-treated mice (white and black bars, respectively). The graph corresponds to the densitometric analysis of each postsynaptic and presynaptic proteins normalized against β-tubulin and compared with the levels of the same protein in AβPP/PS1 control mice. Immunoblots of total protein extracts from the hippocampi of 12-month-old AβPP/PS1 control and ANDRO-treated mice (white and black bars, respectively). (b) Immunoblots of total postsynaptic proteins (PSD-95, GluA2, Shank and GluN2B) and presynaptic proteins (SYP and VAMP) extracts from the hippocampi of 12-month-old AβPP/PS1 control and ANDRO-treated mice (white and black bars, respectively). Graph corresponds to the densitometric analysis of each postsynaptic and presynaptic proteins normalized against β-tubulin and compared with the levels of the same protein in AβPP/PS1 control mice, n ≥ 3. *p < 0.05; **p < 0.01; ***p < 0.001. (c) Immunoblots of total GSK-3β, inactive form of GSK-3β (pGSK3βser9) and active form of GSK-3β (pGSK3βtyr216) proteins extracts from the hippocampi and cortex of 12-month-old AβPP/PS1 control and ANDRO-treated mice (white and black bars, respectively). (d) Immunoblots of total β-catenin and activated form of β-catenin proteins extracts from the hippocampi and cortex of 12-month-old AβPP/PS1 control and ANDRO-treated mice (white and black bars, respectively) Graph corresponds to the densitometric analysis of each postsynaptic and presynaptic proteins normalized against actin and compared with the levels of the same protein in AβPP/PS1 control mice, n ≥ 3. *p < 0.05; **p < 0.01; ***p < 0.001.

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