Figure 5

ANDRO increases the synaptic transmission and protect the LTP against the Aβ oligomers in vitro. (a) fEPSP recordings in the presence of 10 μM ANDRO. (b) Plot of paired pulse facilitation (PPF) in the presence or absence of ANDRO. Inset shows representative recordings. Bars represent the mean ± SE from 7 different slices, *p < 0.05. (c) fEPSP amplitude induced by the input–output protocol treated with ANDRO (black circle) or with vehicle solution (ACSF, white circles). (d) Fiber volley (FV) amplitude induced by the input–output protocol treated with ANDRO (black circle) or with vehicle solution (white circles). (e) Hippocampal slices were exposed to ANDRO, and LTP was induced. The arrow indicates LTP induction by TBS, and the plot shows the fEPSP slope at different times. (f) Hippocampal slices were exposed to Aβ oligomers (1 μM); arrow indicates the time of TBS and the plot show the fEPSP slope at different times. (g) Hippocampal slices were exposed to ANDRO (10 μM) in the presences of Aβ oligomers (1 μM), arrow indicates the time of TBS and the plot show the fEPSP slope at different times. (h) Plot of fEPSP slope changes, in the presence or absence of ANDRO plus Aβ oligomers. The inset shows representative recordings. The dots and bars represent the mean ± SE from 7 different slices, *p < 0.05. Three animals were used per experimental group. Data are presented as mean ± SEM. Statistical differences were calculated by Student’s t test, followed by Dunnett’s post hoc test. Asterisks indicate statistically significant differences (*p < 0.05). Statistical significant differences in in vitro experiments of ANDRO and Aβ oligomers were calculated by one-way ANOVA, followed by Bonferroni’s post hoc test.