Figure 6

ANDRO inhibits GSK-3β, increases β-catenin levels and inhibits LTD induction. (a) Hippocampal slices from wild-type animals were exposed to different concentrations of ANDRO (0.1, 5 and 10 μM, black circles) to inhibit the LTD in the CA1 hippocampal region. The arrow indicates the time of LFS, and the plot shows the fEPSP slope at different times. (b) The right graph represents the quantification of the LTD magnitude to 60 min post-induction in the presence of ANDRO (black bars). (c) Hippocampal slices from wild-type mice were exposed to 6-BIO (10 nM), and LTD was induced. The arrow indicates the time of LFS, and the plot shows the fEPSP slope at different times. The inset shows representative recordings. (d) Plot of changes in fEPSP slope in the presence or absence of 6-BIO. (e) Quantification shows that the presence of ANDRO (black bars) induces an increase in the phosphorylation of GSK-3β (pGSK3βser9) and a reduction in pGSK3βtyr216 compared with ACSF treatment (white bars). Each protein was normalized against total GSK-3β. (f) β-catenin was evaluated in the presence of ANDRO (black bars) or ACSF (white bars). Densitometric analysis of each protein normalized against total β-catenin and β-actin (loading control) were compared with the levels of the same protein in hippocampal slices from wild-type animals. Bars represent the mean ± SE n ≥ 3. *p < 0.05; **p < 0.01. The inset shows representative recordings. The dots are the mean ± SE from 7 different slices, n ≥ 3. * p < 0.05. Three animals were used per experimental group. Data are presented as mean ± SEM. Statistical differences were calculated by Student’s t test, followed by Dunnett’s post hoc test.. Statistical significant differences in dosis response were calculated by one-way ANOVA, followed by Bonferroni’s post hoc test.