Figure 10
From: Effects of the C57BL/6 strain background on tauopathy progression in the rTg4510 mouse model
Increased PHF1 staining in older NT B6 mice compared to NT mice. NT mice on an F1 FVB/B6 background (NTB6) had increased PHF1 (pS396/404 tau) staining in the cortex, hippocampus and brainstem regions of the brain compared to NT mice on an F1 FVB/129 background (NT). (A) Representative images from the primary sensory cortex, hippocampal formation and brainstem of NTB6 and NT mice stained with PHF1. (B) Quantification of PHF1 burden in the cortex, hippocampus, and brainstem of 6.5- and 10.5-month-old NTB6 and NT mice. Cortical PHF1 staining was significantly increased in NTB6 mice compared to NT mice only at 10.5 months of age, while at 6.5 and 10.5 months of age PHF1 burden was increased in the hippocampal formation and brainstem. Each triangle represents an individual mouse with the mean indicated by the black line, n = 3-7 per cohort. ***p < 0.0001 [two-way ANOVA (strain x age): main effect of strain indicated]. ###p < 0.001 (post hoc Bonferonni’s multiple comparisons test). Scale bars represent 200 μm. CC = Corpus callosum; 7 N = 7th Cranial nerve.