Figure 3
From: Effects of the C57BL/6 strain background on tauopathy progression in the rTg4510 mouse model
Equivalent accumulation and phosphorylation of insoluble tau in 2.5-month-old rTg4510 B6 and rTg4510 mice. (A-B) Western blot analyses of the sarkosyl-insoluble fractions of whole brain lysates from rTg4510B6 and rTg4510 mice at 2.5 months of age. (A) Representative western blots of insoluble human tau (E1 antibody) and phosphorylated tau (CP13 and PHF1 antibodies) with a NT littermate shown as a negative control. (B) Densitometric quantification of E1 (human tau), CP13 (pS202 tau) and PHF1 (pS396/404 tau). Phospho-tau is normalized to the amount of human tau aggregated in that fraction. Insoluble tau accumulation and phosphorylation were similar between rTg4510B6 and rTg4510 mice. Each dot represents an individual mouse with the mean indicated by the black line. n = 9-10 per cohort.