Figure 4

Age-dependent decline in post-synaptic protein levels is exacerbated in 5xFAD/ APOE -KO ≥ E4FAD mice compared to E3FAD and E2FAD mice. Results at 2-, 4-, and 6-months of age for E2FAD, E3FAD, E4FAD, and 5xFAD/APOE-KO mice: (A) Representative Western blot images for PSD95, drebrin and synaptophysin proteins in HP with β-actin as a control for protein loading (APOE-KO = 5xFAD/APOE-KO). Relative protein levels of (B) synaptophysin, (C) PSD95 and (D) drebrin. N = 6 per group, expressed as means ± S.E.M. Significant differences at p < 0.05 via two-way ANOVA, Bonferroni post-hoc test identified by: ^†between E2FAD and E3FAD, § between E2FAD and E4FAD, ^¶between E3FAD and E4FAD. Color matched ^‡(green = E2FAD, blue = E3FAD, red = E4FAD, grey = 5xFAD/APOE-KO) between EFAD strain and 5xFAD/APOE-KO. Along the X-axis, color matched *indicates significant differences between time points within a mouse strain. There is no significant change with age unless marked.