Figure 6

Age-dependent decline in NMDAR-mediated signaling proteins is exacerbated in E4FAD ≥ 5xFAD/ APOE -KO ≥ E3FAD > E2FAD mice. Results at 2-, 4-, and 6-months of age for E2FAD, E3FAD, E4FAD, and 5xFAD/APOE-KO mice: (A) Representative Western blot images for p-CaMK-II, p-CREB, and BDNF proteins in HP with β-actin/CREB, β-actin/CaMK-II or β-actin as a control for protein loading, respectively (APOE-KO = 5xFAD/APOE-KO). Relative protein levels of (B) p-CaMK-II, (C) p-CREB and (D) BDNF. N = 6 per group, expressed as means ± S.E.M. Significant differences at p < 0.05 via two-way ANOVA, Bonferroni post-hoc test identified by: ^†between E2FAD and E3FAD, ^§between E2FAD and E4FAD, ^¶between E3FAD and E4FAD. Color matched ^‡(green = E2FAD, blue = E3FAD, red = E4FAD, grey = 5xFAD/APOE-KO) between EFAD strain and 5xFAD/APOE-KO. Along the x-axis, color matched *indicates significant differences between time points within a mouse strain. There is no significant change with age unless marked.