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Figure 6 | Molecular Neurodegeneration

Figure 6

From: Intrathecal heat shock protein 60 mediates neurodegeneration and demyelination in the CNS through a TLR4- and MyD88-dependent pathway

Figure 6

Compared to LPS, intrathecal HSP60 does not induce considerable inflammation in the CNS. (A) C57BL/6 J mice were injected intrathecally with 40 μg HSP60 (n = 4), 40 μg SA (control, n = 4), or 10 μg LPS (n = 4). After 6 h, 12 h, and 72 h brain lysates were analyzed for the expression of various genes encoding proinflammatory molecules, as indicated, by qPCR. Normalized values (ΔCT) per animal with the median of the respective group; *p < 0.05, **p < 0.005, ***p < 0.001; ANOVA of log2 transformation followed by Bonferroni-selected pairs; versus SA. Fold increase to SA was calculated with the median. (B) C57BL/6 J mice were injected intrathecally with 40 μg HSP60 (n = 4), 40 μg SA (control, n = 4), or 10 μg LPS (n = 4). Sham-operated animals served as a further control group (n = 4). After 12 h brain lysates were analyzed for the expression levels of proinflammatory proteins, as indicated, by multiple analyte detection and Il-1β ELISA. Median; *p < 0.05, **p < 0.005; Kruskal-Wallis followed by Dunn’s selected pairs; versus sham. (C, D) C57BL/6 J mice received sham surgery (n = 4) or were injected intrathecally with 40 μg HSP60 (n = 4), 40 μg SA (n = 4), or 10 μg LPS (n = 4). After 12 h brain lysates were analyzed for (C) mRNA expression of various enzymes associated with the production of neurotoxic metabolites, as indicated, and (D) the content of nitrite (NO). (C) Levels of mRNA expression were determined by qPCR and expressed as ΔCT per animal with the median; ***p < 0.001; ANOVA of log2 transformation followed by Bonferroni-selected pairs; versus sham. (D) Determination of the amount of NO by Griess’ reaction; Median, Kruskal-Wallis test followed by Dunn’s selected pairs.

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