Figure 4From: Posttranslational modification and mutation of histidine 50 trigger alpha synuclein aggregation and toxicity SDGC analysis of recombinant WT, H50Q, and H50R aSyn. Recombinant human WT aSyn is clearly detected in the low density factions from 16 to 22 (row 1 of the upper panel). Oligomerized aSyn is characterized by a signal shift towards higher density fractions, which is exemplarily displayed by HNE-aSyn and n-aSyn consisting of oligomeric aSyn (rows 1 and 2 of the lower panel). Large fibrillar aggregates appear in the first exclusion fractions (row 3 of the lower panel). H50 mutations (H50Q and H50R) increase aSyn immunosignal in the 60% high density exclusion fraction (arrows, rows 2 and 3 of the upper panel).Back to article page