Figure 5

Nitration-induced oligomerization of WT aSyn and aSyn H50 mutants. A) Recombinant WT aSyn and aSyn H50 mutants were treated with 5 mM TNM for 7 h to induce nitration-mediated oligomerization of aSyn. Formation of soluble aSyn oligomers after TNM treatment was assessed by SEC. aSyn monomers (M) and oligomers (O) are distinguished by different retention times. B) Quantification of soluble oligomers was performed by calculating the area ratio of oligomeric fraction to monomeric fraction. H50 mutations increase the formation of soluble oligomers after TNM treatment. Particularly, the H50Q mutation significantly enhances the propensity of nitration-mediated aSyn oligomerization. Significance was determined by a one way ANOVA Tukey’s Multiple Comparison Test. n = 3. Error bars = SEM.