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Figure 2 | Molecular Neurodegeneration

Figure 2

From: An automated image analysis method to measure regularity in biological patterns: a case study in a Drosophila neurodegenerative model

Figure 2

Representation of image processing steps performed by FLEYE plugin. Starting with eye surface images (A) a “training step” is performed where the plugin detection is fitted to the number of user-counted ommatidia in WT eyes (B). Then, the user defines a region of interest (ROI) (C). An averaging step (Filter) normalizes the picture (D) and pixel maxima are identified (E). A squared grid is applied to the single-pixel maxima image (F). The final variables are obtained either globally from the distance between maxima or locally from spatial information of the pixel distribution in every cell of the grid.

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