Figure 2

Altered clock molecule expression in the SCN of 5XFAD mice. (A) Temporal changes in mRNA levels of clock genes in the SCN of 5XFAD mice. The total RNA of the SCN was prepared at 4 h intervals. The mRNA levels of Bmal1, Cbp and Per2 were quantified using real-time PCR. Data are represented as mean ± SEM; ^* P < 0.05, ^** P < 0.01, ^*** P < 0.001. (B) Temporal changes in the protein levels of clock proteins in the SCN of 5XFAD mice. Brain samples were prepared at 4 h intervals. Western blot analysis revealed that BMAL1, CBP and PER2 protein oscillations were decreased in 5XFAD mice. (C) Densitometric analysis of BMAL1, CBP and PER2 protein expression from three independent experiments. BMAL1, CBP and PER2 were normalized to α-Tubulin. Data are represented as mean ± SEM. ^* P < 0.05, ^** P < 0.01.