Immunization with AFF 1 reduces α-syn accumulation in MBP-α-syn tg mice. (A) α-syn immunoreactivity in neocortex and striatum of non-tg and MBP-α-syn tg mice immunized with vehicle, original C-terminal α-syn antigen, or AFF 1 (B) Cell counts of α-syn-positive cells in neocortex (C) Cell counts of α-syn-positive cells in striatum (D) Immunoblot analysis of total α-syn in the cytosolic and membrane fractions of protein extracts from non-tg and MBP-α-syn tg mice immunized with vehicle, α-syn antigen, or AFF 1. Significant results of two mice per group are shown (E) Densitometric analysis of the α-syn immunoreactive bands in the cytosolic fraction (F) Densitometric analysis of the α-syn immunoreactive bands in the membrane fraction. Results are expressed as average ± SEM. (#) p < 0.05 when comparing vehicle-treated tg animals vs. immunized tg animals by one-way ANOVA with post hoc Tukey-Kramer. For the vehicle-treated non-tg group, n = 5. For tg groups, n = 10 animals per group.