Figure 4

Immunization with AFF 1 promotes microglial activation in MBP-α-syn tg mice. Non-tg mice or MBP-α-syn tg mice were immunized with vehicle, original C-terminal α-syn antigen or AFF 1, and glial markers were analyzed by immunohistochemistry. (A) Immunostaining of the astroglial marker GFAP in neocortex and striatum. Scale bar = 25 μm (B) Optical density quantification of GFAP staining in neocortex (C) Optical density quantification of GFAP staining in striatum (D) Immunostaining of the microglial marker Iba1 in neocortex and striatum. Scale bar = 25 μm (E) Quantification of Iba1-positive cell counts in neocortex (F) Quantification of Iba1 cell counts in striatum. Results are expressed as average ± SEM. (*) p < 0.05 when comparing vehicle-treated non-tg animals to tg groups by one-way ANOVA with post hoc Dunnett; (#) p < 0.05 when comparing vehicle-treated tg animals with immunized tg groups by one-way ANOVA with post hoc Tukey-Kramer. For the non-tg group, animal numbers were n = 5 for vehicle and n = 3 for AFF 1. For tg groups, n = 10 animals per group.