Immunization with AFF 1 reduces demyelination in MBP-α-syn tg mice. (A) LFB staining of myelin in the neocortex (and corpus callosum) and striatum of non-tg mice or MBP-α-syn tg mice immunized with vehicle, original C-terminal α-syn antigen or AFF 1. Scale bar = 250 μm (B) Quantification of LFB staining by optical density in neocortex (C) Quantification of LFB staining by optical density in striatum (D) Electron microscopy images of myelin sheaths in the corpus callosum of non-tg mice or MBP-α-syn tg mice immunized either with vehicle, α-syn antigen or AFF 1. Representative images taken with the transmission electron microscope (TEM) are shown at low magnification (5,000x) and high magnification (25,000x). Scale bars = 2.5 μm and 500 nm. vac, vacuola (E) Quantification of the number of myelinated axons in corpus callosum (F) Quantification of the average number of myelin layers per axon in corpus callosum. Results are expressed as average ± SEM. (*) p < 0.05 when comparing vehicle-treated non-tg animals to tg groups by one-way ANOVA with post hoc Dunnett; (#) p < 0.05 when comparing vehicle-treated tg animals with immunized tg groups by one-way ANOVA with post hoc Tukey-Kramer. For the non-tg group, animal numbers were n = 5 for vehicle and n = 3 for AFF 1. For tg groups, n = 10 animals per group.