Skip to main content
Figure 2 | Molecular Neurodegeneration

Figure 2

From: Lazarillo-related Lipocalins confer long-term protection against type I Spinocerebellar Ataxia degeneration contributing to optimize selective autophagy

Figure 2

Rescue of hATXN1 82Q -dependent photoreceptor degeneration by GLaz over-expressed with a native spatiotemporal pattern. A, Representative examples of scanning electron microscopy and magnified images of retinas degenerated (gmrā€‰>ā€‰hATXN182Q), and co-expressing different GLaz expression transgenes (glaz:GLaz-GFP[FX] and glaz:GLaz-GFP[F2]). B, Quantitative estimate of degeneration by computing a regularity index based on the variance (Ļƒ) of the local intensity maxima distances. A percent recovery of the degeneration is indicated for each genotype. 20ā€“35 eyes/genotype were used to compute regularity indexes. Averageā€‰Ā±ā€‰S.E.M. are represented. Asterisk represents statistically significant differences (Studentā€™s t-test, *Pā€‰<ā€‰0.05) with respect to the degenerated gmrā€‰>ā€‰hATXN182Q genotype. C, a,b: GLaz expression revealed by GFP fluorescence immunohistochemistry of paraffin sections of retinas of flies expressing hATXN182Q and the transgene glaz:GLaz-GFP[FX]. Nuclei are shown with DAPI staining. c,d: Double fluorescence immunohistochemistry revealing GLaz and the photoreceptor marker rhodopsin (4C5). DAPI stains nuclei in C.

Back to article page