Rescue of hATXN1
-dependent photoreceptor degeneration by GLaz over-expressed with a native spatiotemporal pattern. A, Representative examples of scanning electron microscopy and magnified images of retinas degenerated (gmr > hATXN182Q), and co-expressing different GLaz expression transgenes (glaz:GLaz-GFP[FX] and glaz:GLaz-GFP[F2]). B, Quantitative estimate of degeneration by computing a regularity index based on the variance (σ) of the local intensity maxima distances. A percent recovery of the degeneration is indicated for each genotype. 20–35 eyes/genotype were used to compute regularity indexes. Average ± S.E.M. are represented. Asterisk represents statistically significant differences (Student’s t-test, *P < 0.05) with respect to the degenerated gmr > hATXN182Q genotype. C, a,b: GLaz expression revealed by GFP fluorescence immunohistochemistry of paraffin sections of retinas of flies expressing hATXN182Q and the transgene glaz:GLaz-GFP[FX]. Nuclei are shown with DAPI staining. c,d: Double fluorescence immunohistochemistry revealing GLaz and the photoreceptor marker rhodopsin (4C5). DAPI stains nuclei in C.