Figure 3

NLaz is over-expressed in response to hATXN1 ^82Q -dependent photoreceptor degeneration, and its over-expression with the GAL4/UAS system rescues the pathogenic phenotype. A, RT-qPCR levels of NLaz mRNA in 5–6 day old fly heads. Statistical differences were assayed by Mann–Whitney U-test. *P < 0.05. B, Representative fly eyes and hematoxilin/eosin histochemistry of paraffin sections showing the degenerated phenotype (gmr > hATXN1^82Q) and the NLaz rescue (gmr > hATXN1^82Q + NLazC5). Arrows mark the retina thickness in the histological sections. C, Photoreceptor degeneration rescue observed when co-expressing hATXN1^82Q with the Lipocalins NLaz and GLaz. D, Quantification of photoreceptor degeneration rescue for NLaz and the co-expression of NLaz and GLaz. Asterisk represents statistically significant differences (Student’s t-test, *P < 0.05) with respect to the degenerated gmr > hATXN1^82Q genotype.