Effects of GLaz over-expression on cell death due to hATXN1
-dependent degeneration, and on SCA1-modifiers expression. A, TUNEL analysis of apoptotic cell death in paraffin sections of degenerated (gmr > hATXN182Q) and GLaz rescued (gmr > hATXN182Q + GLaz2) retinas. Representative examples of retina sections showing a decreased number of apoptotic nuclei (white arrows) in flies that over-express GLaz. B, hATXN1 mRNA levels in fly heads of the SCA1 model and lines expressing a double dose of GLaz. C, RT-qPCR expression levels of the SCA1 neurodegeneration modifiers GstS1 and Vib. Statistical differences of RT-qPCR data were assayed by Mann–Whitney U-test. *P < 0.05.