Effects on Atg8a and p62 expression of GLaz loss- and gain-of-function in control conditions. A-B, Immunofluorescence of control (either WTG10 or gmr > +), GLaz loss-of-function (GLazΔ2/Δ2) and GLaz gain-of-function (gmr > GLaz2) retinas showing p62 protein expression. C, mRNA levels of p62, measured by RT-qPCR in control and GLaz loss- and gain-of-function fly heads. D, mRNA levels of Atg8a, measured by RT-qPCR in control, GLaz loss- and gain-of-function fly heads. Statistical differences were assayed by Student’s t-test (immunofluorescence) and Mann–Whitney U-test (RT-qPCR). *P < 0.05.