Table 1 RT-qPCR primer sequences
Gene | Primer orientation | Primer sequence |
|---|---|---|
NLaz | Forward | 5′-CGAGTACGCAGCCTATCCAT-3′ |
Reverse | 5′-CCAGGTAGTTGGCCTTCGT-3′ | |
GLaz | Forward | 5′-GCGAACAATCGAAGTTTTCC-3′ |
Reverse | 5′-ACAAGATGGCGAAGTTCTCG-3′ | |
hATXN1 | Forward | 5′-GTGGCCGTGATACAGTTCG-3′ |
Reverse | 5′-AGCCGTTCTTCAGGTTCTTG-3′ | |
GstS1 | Forward | 5′-AAGGACAACGATGGTCACCTGGC-3′ |
Reverse | 5′-CGGTGAACTTAGACCTCGGTGACG-3′ | |
Vib | Forward | 5′-GGCCAATCGCACTCCCCAGTTC-3′ |
Reverse | 5′-TAACACCTCGATGCCCTCGCC-3′ | |
Atg8a | Forward | 5′-CATCGGTGATTTGGACAAGA-3′ |
Reverse | 5′-AGTCCTCCTCGTGATGTTCC-3′ | |
p62 | Forward | 5′-CGTAAGGACCTTCTGGATCG-3′ |
Reverse | 5′-CGTCGTGGATGGTGAAATTG-3′ | |
L18 | Forward | 5′-AGAACCGAGCCCAAATCC-3′ |
Reverse | 5′-CGACCACGATGGTAGACTCC-3′ |