Figure 1

Western blot analysis of various tau species in brain lysates from Tg4510 mice of different ages. Soluble and insoluble fractions of brain homogenates were prepared as described in Methods from rTg4510 mice that were 6, 16 or 32 weeks of age. Ten μg of protein from each fraction was analyzed by probing Western blots with the specified antibodies. The position of the 55 kD and 64 kD species of tau are indicated. α-Tubulin was used as a loading control. A. Total tau detected by two different antibodies (HT7 and Tau12). B. P-tau species enriched in 64 kD tau in the insoluble fraction. C. P-tau species present in both the 55 kD species in the soluble fraction and in the 64 kD species in the insoluble fraction.