Figure 2
From: Analysis of tau post-translational modifications in rTg4510 mice, a model of tau pathology
Age-dependent change of total tau and tau aggregates in the brain insoluble fraction of rTg4510 mice. Brain insoluble fractions from animals 6–32 weeks of age (n = 18-20 per group) were analyzed using AlphaLISA based immunoassays. A. Total tau measured in an immunoassay using biotin-Tau12 for capture and HT7-conjugated acceptor beads for detection. Twenty ng of protein from the insoluble fractions were used per assay. Recombinant tau (Millipore) was used to generate a standard curve for quantification. B. Tau aggregates measured in an immunoassay using HT7 as both capture and detection antibody. Two hundred ng of protein from the insoluble fractions were used in each assay. The Y-axis is the relative fluorescence readout from Envision (PerkinElmer). Compared to 8-week old mice, total tau and tau aggregate changes reached statistical significance after 14-, and 16-weeks of age, respectively (p <0.05, one-way ANOVA).