Figure 3
From: Analysis of tau post-translational modifications in rTg4510 mice, a model of tau pathology
Age-dependent change of p-tau in the brain insoluble fraction of rTg4510 mice. Brain insoluble fractions from animals 6–32 weeks of age (n = 18-20 per group) were analyzed using AlphaLISA based immunoassays. PHF6 tau (A), AT8 tau (B), and pThr global tau phosphorylation (C) were measured using biotin-HT7 for capture and specific p-tau antibodies for detection. Two hundred ng of protein from the insoluble fractions were used in each assay. The Y-axis is the relative fluorescence readout from Envision (PerkinElmer). Compared to 8-week old mice, PHF6, AT8 and pThr tau changes reached statistical significance after 16-, 20-, and 16-week of age, respectively (p <0.05, one-way ANOVA).