Overexpression of normal or mutant CREST induces retinal degeneration in
(A) Protein expression levels of CREST variants in the heads of transgenic flies of each line as determined by Western blotting and subsequent quantification of band intensities (mean ± s.d). (B) Images of external head surface of 5-day-old flies overexpressing wild-type CREST, CREST I123M, CREST Q388stop or lacZ in the retina. Overexpression of wild-type CREST or its variants in photoreceptor neurons results in rough and depigmented eyes. (C) H&E staining of retinal sections of 5-day-old flies overexpressing wild-type CREST or its variants reveals disruption of regularly ordered arrays of photoreceptor neurons. Data for representative transgenic fly lines with similar (intermediate) level of CREST protein expression are shown (#7, #7 and #2 for WT, I123M and Q388stop variants, respectively, – see Additional file 5: Figure S4). Scale bar, 50 μm.