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Table 1 Experimental design

From: Nigral overexpression of alpha-synuclein in the absence of parkin enhances alpha-synuclein phosphorylation but does not modulate dopaminergic neurodegeneration

  Group
Analysis time point (Immunohistochemistry) rAAV2/7-WT α SYN rAAV2/7-eGFP rAAV2/7-WT α SYN
(4E + 11 GC/ml) (4E + 11 GC/ml) (1E + 11 GC/ml)
high α -SYN dose   low α -SYN dose
parkin +/+ parkin −/− parkin +/+ parkin −/− parkin +/+ parkin −/−
1 week n = 4 n = 4    n = 2 n = 3
4 weeks n = 15 n = 16 n = 5 n = 6 n = 10 n = 13
8 weeks      n = 6 n = 5
16 weeks      n = 6 n = 6
  1. Adult 2 to 4-month-old parkin+/+ and parkin−/− mice were stereotactically injected in the right SN with 2 μl of rAAV2/7-WT α-SYN vector at a titer of 4E + 11 GC/ml (high α-SYN dose) or 1E + 11 GC/ml (low α-SYN dose) or rAAV2/7-eGFP vector at a titer of 4E + 11 GC/ml. At the mentioned time points after injection, animals were perfused for immunohistochemical analysis.