Figure 3
From: Making (anti-) sense out of huntingtin levels in Huntington disease
HTT mRNA quantification after RT-PCR amplification across the CAG repeat compared to SNP-specific quantitative RT-PCR. Whisker boxplots of wild-type versus mutant HTT mRNA expression levels in adult-onset HD post-mortem brain material. (A) Quantification after amplification across the CAG repeat, relative to gDNA. Pair wise differences were evaluated using linear mixed model, n = 10. (B) SNP rs362273-specific quantitative RT-PCR, normalized to β-actin (ACTB). Data were evaluated using a two-tailed student t-test, n = 4.