Fig. 5
From: Cholestenoic acid, an endogenous cholesterol metabolite, is a potent γ-secretase modulator
CA derivatives produced in the cholesterol elimination pathway decrease Aβ42 levels in CHO-2B7 cells. a Acidic pathway of the cholesterol elimination pathways is illustrated. Sequential hydroxylation on the carbon-23 by Cyp27a1 converts cholesterol to CA, and then Cyp7b1 converts CA to a CA derivative, 7α-OH-CA. 7α-OH-CA is further dehydrogenized by HSD3b7 producing 7α-OH-4-CA. b Dose–response curves were established to determine EC50 for the cholesterol metabolites found in this pathway using the conditioned media from CHO-2B7 cells treated with them. Compared to the DMSO control, CA and its derivatives showed Aβ decreasing effects at different EC50s. CA EC50 was at 250nM, 7α-OH-CA was at 970nM, and 7α-OH-4-CA was at 4150nM. 27-OHC EC50 could not be determined