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Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: Mutations in LRRK2 potentiate age-related impairment of autophagic flux

Fig. 2

Quantification of autophagic flux over the lifespan. a. Representative pictures from the wlz56 dat-1::lgg-1::mCherry and dat-1::GFP lines at varying ages. b. Total mCherry fluorescence from four anterior CEP neurons and the lateral regions of the head ring was captured in age synchronized dat-1::lgg-1::mCherry nematodes over much of the nematode lifespan (N = 15, for each time point). The fluorescence was normalized to corresponding data captured from age-synchronized C. elegans carrying dat-1::GFP to control for differences in the size of DA neurons as the nematodes matured and aged (Fig. C). c dat-1::GFP fluorescence over nematode lifespan. Total GFP fluorescence from all four CEP neurons are plotted to quantify the pattern of dat-1::GFP expression (N = 15, for each time point). The increased fluorescence at young ages reflects the increasing size of the neurons, while the decreased fluorescence at older ages reflects age-related degeneration of the dopaminergic neurons. The mean fluorescence data were used to correct the age and cell size data for lgg1-mCherry expression for Fig. 3

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