Fig. 4

Generation of the dat- 1::lmp-1::GFP C. elegans line. a. Structure of dat-1:: lmp-1::GFP reporter construct. b. Expression of dat-1::lmp-1::GFP construct in dopaminergic neurons of day 3 adult nematodes. The lmp-1::GFP identifies locations of lysosomal vesicles in the anterior deirid (ADE) and CEP dopaminergic neurons and in their dendrites. Scale bar = 40 μm. c. Quantification of lmp-1::GFP fluorescence of the dat- 1::lmp-1::GFP lines and lines crossed to the snb-1::LRRK2 (WT or G2019S) lines. d. Co-expressing the dat- 1::lmp-1::GFP construct with snb::LRRK2 (WT or G2019S) changes GFP fluorescence only marginally while mCherry fluorescence shows robust changes in response to the type of LRRK2 transgene expressed (adult Day 3). Scale bar = 40 μm. The insets (white boxes) are shown in the adjacent column at higher magnification