Fig. 1
From: Brca1 is expressed in human microglia and is dysregulated in human and animal model of ALS
FACS analysis of CD11b+ microglia in control and hSOD1G93A mice from the lumbar segment of the spinal cords at 90 days of age. Microglia were sorted by flow cytometry using the microglia marker CD11b. a Confocal images of CD11b expression in spinal cord microglia from control at 90 day of age and (b) from transgenic hSOD1G93A mice at early symptomatic age. Scale bars (a & b): 50 μm. c–d Representative flow cytometry analysis dot plot displaying microglia profiles. c Control and (d) hSOD1G93A spinal microglia at P90. In both (c) control and (d) hSOD1G93A surrounded areas, designed as “P4”, correspond to the labelled cells. e Negative control (without CD11b labelling). The X-axis represents the intensity of fluorescence and the Y axis the size of the cells. f RNA quality isolated from FACSed microglia