Prophylactic melatonin significantly reduces Alzheimer’s neuropathology and associated cognitive deficits independent of antioxidant pathways in AβPPswe/PS1 mice

Table 1 Gene-specific primers used for qPCR experiments and genotyping

Gene	Gene-specific Primers 5’ – 3’	GenBank# or Primer ID	Amplicon Size	Anneal Temp
SOD1	S: ATG GCG ATG AAA GCG GTG TG	NM_011434	460	59 °C
	AS: GCG CAA TCC CAA TCA CTC CA
GPx-1	S: ATG TGT GCT GCT CGG CTC TC	NM_008160	590	60 °C
	AS: TGC TGG GAC AGC AGG GTT TC
CAT	S: AGG TTT GGC CTC ACA AGG AC	NM_009804	239	58 °C
	AS: GCG GTA GGG ACA GTT CAC AG
Nrf2	S: AAC GAC AGA AAC CTC CAT CTA C	NM_010902	94	57 °C
	AS: AGT AAG GCT TTC CAT CCT CAT C
Rp27	S: CCA GGA TAA GGA AGG AAT TCC TCC TG	NM_024277	297	59 °C
	AS: CCA GCA CCA CAT TCA TCA GAA GG
MT1R	GAG TCC AAG TTG CTG GGC AGT GGA	mMT1R-REV-WT		94 °C
	GAA GTT TTC TCA GTG TCC CGC AAT GG	mMT1R-FW-WT	480
	CCA GCT CAT TCC TCC ACT CAT GAT CTA	mMT1R-NEOFW-KO	240
MT2R	CCA GGC CCC CTG TGA CTG CCC GGG	mMT2R-FW-WT		68 °C
	CCT GCC ACT GAG GAC AGA ACA GGG	mMT2R-REV-WT	272
	TGC CCC AAA GGC CTA CCC GCT TCC	mMT2R-NEO-REV	550
AβPP	AGG ACT GAC CAC TCG ACC AG	oIMR3610	377	52 °C
	CGG GGG TCT AGT TCT GCA T	oIMR3611
Psen1	AAT AGA GAA CGG CAG GAG CA	oIMR1644	608	54 °C
	GCC ATG AGG GCA CTA ATC AT	oIMR1645
Pos Ctrl	CTA GGC CAC AGA ATT GAA AGA TCT	oIMR7338	324	54 °C
	GTA GGT GGA AAT TCT AGC ATC ATC C	oIMR7339

ISSN: 1750-1326