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Table 1 Gene-specific primers used for qPCR experiments and genotyping

From: Prophylactic melatonin significantly reduces Alzheimer’s neuropathology and associated cognitive deficits independent of antioxidant pathways in AβPPswe/PS1 mice

Gene Gene-specific Primers 5’ – 3’ GenBank# or Primer ID Amplicon Size Anneal Temp
SOD1 S: ATG GCG ATG AAA GCG GTG TG NM_011434 460 59 °C
  AS: GCG CAA TCC CAA TCA CTC CA    
GPx-1 S: ATG TGT GCT GCT CGG CTC TC NM_008160 590 60 °C
  AS: TGC TGG GAC AGC AGG GTT TC    
CAT S: AGG TTT GGC CTC ACA AGG AC NM_009804 239 58 °C
  AS: GCG GTA GGG ACA GTT CAC AG    
Nrf2 S: AAC GAC AGA AAC CTC CAT CTA C NM_010902 94 57 °C
  AS: AGT AAG GCT TTC CAT CCT CAT C    
Rp27 S: CCA GGA TAA GGA AGG AAT TCC TCC TG NM_024277 297 59 °C
  AS: CCA GCA CCA CAT TCA TCA GAA GG    
MT1R GAG TCC AAG TTG CTG GGC AGT GGA mMT1R-REV-WT   94 °C
  GAA GTT TTC TCA GTG TCC CGC AAT GG mMT1R-FW-WT 480  
  CCA GCT CAT TCC TCC ACT CAT GAT CTA mMT1R-NEOFW-KO 240  
MT2R CCA GGC CCC CTG TGA CTG CCC GGG mMT2R-FW-WT   68 °C
  CCT GCC ACT GAG GAC AGA ACA GGG mMT2R-REV-WT 272  
  TGC CCC AAA GGC CTA CCC GCT TCC mMT2R-NEO-REV 550  
AβPP AGG ACT GAC CAC TCG ACC AG oIMR3610 377 52 °C
  CGG GGG TCT AGT TCT GCA T oIMR3611   
Psen1 AAT AGA GAA CGG CAG GAG CA oIMR1644 608 54 °C
  GCC ATG AGG GCA CTA ATC AT oIMR1645   
Pos Ctrl CTA GGC CAC AGA ATT GAA AGA TCT oIMR7338 324 54 °C
  GTA GGT GGA AAT TCT AGC ATC ATC C oIMR7339