Fig. 1

Tau hyperphosphorylation in the brain of Akt cTKO mice. a Western blotting of p-tau using cortical and cerebellar samples. Antibodies against tau phosphorylated at the Thr205, Thr231 and Ser396 epitopes were used. The migration of three p-tau bands (60 kDa, 64 kDa and 68 kDa) in SDS gels was slower in Akt cTKO (Akt1 ^f/f ;Akt2 ^−/− ;Akt3 ^−/− ;CAG-CreER with tamoxifen treatment) than in control mice (Akt1 ^f/f ;Akt2 ^+/+ ;Akt3 ^+/+ and Akt1 ^f/+ ;Akt2 ^+/+ ;Akt3 ^+/+). Intensities of the p-tau bands were dramatically increased in Akt cTKO mice. Tau5 antibody was used to detect total tau (t-tau). b Immunohistochemistry of p-tau^Thr205 in the brain. Strong immuno-reactivity of p-tau^Thr205 was seen in the cortex (e), hippocampal CA3 (f), the hilus of the dentate gyrus (g) and the cerebellum (h) of Akt cTKO mice. Weak immuno-reactivity of p-tau^Thr205 was observed in different brain sub-areas of control mice (a-d)