Fig. 3
From: Conditional inactivation of Akt three isoforms causes tau hyperphosphorylation in the brain
Increased levels for an activated form of GSK3α in the brain of Akt cTKO mice. a Western blotting on activated forms of GSK3α and GSK3β. Quantitative analysis showed significantly increased levels for p-GSK3αTyr279 (*, p < 0.05) but unchanged levels for p-GSK3βTyr216 (NS, p > 0.05) in Akt cTKO mice. b Western blotting on p-Cdk5, p35 and p25. Relative levels for p-Cdk5Ser159 were significantly increased (*, p < 0.05), but those for p25 were significantly decreased (*, p < 0.05) in the cortex of Akt cTKO mice. GAPDH served as the loading control. c Western blotting on MAPK Erk1/2. Total Erk1/2 levels in Akt cTKO mice were not different from those in control mice. β-actin served as the loading control. Ratio of p-Erk1/2 to the total Erk1/2 in Akt cTKO mice was also not different from that in control mice (p > 0.05). d Western blotting on phosphorylated MAPK p38 (p-p38). Levels of total p38 did not differ between Akt cTKO and control mice (p > 0.05). Ratio of p-p38 to total p38 in Akt cTKO mice was not changed (NS = not significant)