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Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: CRISPR/Cas9: a powerful genetic engineering tool for establishing large animal models of neurodegenerative diseases

Fig. 1

CRISPR-Cas9 targeting system. In the CRISPR/Cas9 system, a guide RNA hybridizes a 20-nt DNA sequence immediately preceding an NGG DNA motif (protospacer-associated motif or PAM), resulting in a double-strand break (DSB) 3 bp upstream of the NGG. The double-stranded DNA breaks become substrates for endogenous cellular DNA repair machinery that catalyze nonhomologous end joining (NHEJ) or homology-directed repair (HDR). Adopted from Charpentier & Doudna, Nature, 2013,495:50–1

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