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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: MicroRNA-9 induces defective trafficking of Nav1.1 and Nav1.2 by targeting Navβ2 protein coding region in rat with chronic brain hypoperfusion

Fig. 3

miR-9 regulates SCN2B evaluated by luciferase array. a, Complementarity between miR-9 seed-matched sequence and the region coding for Navβ2 predicted by a computational and bioinformatics-based approach using RNA22/RNAhybrid. Two binding sites were found at the position of 336–358 of SCN2B CDS and the position of 575–597 of SCN2B CDS. The mutation made to genes are underlined. b, Luciferase reporter gene assay for interactions between miR-9 and its binding sites in the CDS region of the Navβ2 mRNA in HEK293T cells. Cells were transfected with luciferase-target motif chimeric vector alone, miR-9, AMO-9, or scramble negative control (NC) using lipofectamine 2000. **P < 0.01 vs NC, mean ± s.e.m. NC-inhibitors means scramble negative inhibitor control, n = 3. c, Luciferase reporter gene assay for interactions between miR-9 and mutation of binding site in the CDS region of SCN2B in HEK293T cells, **P < 0.01 vs NC, mean ± s.e.m, n = 3

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