Fig. 1

Synaptotoxic effects of amyloid oligomers in primary neuronal cultures. a Primary hippocampal neuronal cultures exposed to Aβ40, Aβ42 or vehicle treated (Ctrl). The cultures were fixed and stained for synaptic marker Synapsin I (green) and neuronal marker MAP2 (red). Scale bar corresponds to 20 μm. b Mean fluorescent intensities of synapsin I staining were divided by mean fluorescent intensities of MAP2 staining for hippocampal cultures treated with Aβ40, Aβ42 or vehicle treated (Ctrl). Average results from three independent experiments are shown. Values are shown as mean ± SEM. *: p < 0.05 by ANOVA one-way test