Fig. 4

STIM2 overexpression protects mushroom spines from amyloid toxicity in vitro. a Primary hippocampal cultures were co-transfected with TD-Tomato and mSTIM2 plasmids or transfected with TD-Tomato. After transfection cells were treated with Aβ42 oligomers or vehicle (Ctrl). Spine morphology was visualized by confocal imaging. Scale bar corresponds to 5 μm. Mushroom spines are indicated with an arrow. b Percentages of mushroom spines (MS) in hippocampal cultures co-transfected with TD-Tomato and mSTIM2 or transfected with TD-Tomato. Data collected from the three batches of cultures are shown for vehicle treated cultures (Ctrl) and cultures treated with Aβ42 oligomers. For spine quantification n = 6–8 (for each treatment per one experiment) neurons were analyzed. Experiment was repeated three times. Values are shown as mean ± SEM. *: p < 0.05 by t-test