Fig. 7

hNSC transplantation increases synaptic density and decreases apoptosis in the NSE/APPsw transgenic mouse brain. a-h Photomicrographs of SVP and PSD95 immunostaining (green and red puncta, respectively) in the posterior parietal cortex (a-d) and striatum radiatum of the hippocampal CA1 region (e-f) in vehicle-injected (Veh; a, c, e, and g) and hNSC-injected (NSC; b, d, f, and h) transgenic mice at 7 weeks post-transplantation. Scale bar, 25 μm (h). i and j Results of image analyses using confocal microscopy for synaptic density (SVP and PSD95) in the cortex (CTX) and hippocampus (HIPP) comparing hNSC- and vehicle-injected transgenic mice (n = 4 per group; I, n = 3 per group; j). k and l Western blot analysis of SVP (k) and PSD95 (l) in both groups (n = 3 per group). m and n Representative images of TUNEL⁺ cells in the posterior parietal cortex of vehicle-injected (m) and hNSC- injected (n) transgenic mice at 7 weeks post-transplantation. Scale bar, 100 μm (h). o Quantification of the number of TUNEL⁺ cells of the posterior parietal cortex in both groups (n = 3 per group). p The relative level of active caspase-3 in the whole brains of hNSC- and vehicle-injected mice on western blot analysis (n = 4 per group). The number of mice (n) in (i-l, o, and p) is indicated. All data represent mean ± SEM. Error bars indicate ± SEM. *p < 0.05