Fig. 3

Expression of transgenic TDP-43 in the pig brain tissues. a Western blot analysis the brain cortex and spinal cord tissues of several transgenic TDP-43 pigs using antibodies to TDP-43 and flag. C: non-transgenic. b Comparison of the transgenic TDP-43 expression in different brain and peripheral tissues in TG-11 and TG-18 pigs. Bracket indicates degraded fragments of transgenic TDP-43. CTX, cortex; CB, cerebellum; LSC, lumbar spinal cord; WM, white matter; BS, brain stem; STR, striatum; HP, hippocampus; SC, spinal cord. Right panel shows quantification of the ratios of endogenous pig TDP-43 to actin in non-transgenic (Control) pig brains and transgenic TDP-43 to actin on western blots (mean ± SE, n = 5 pigs per group). c The brain cortex from non-transgenic (Control) and TDP-43 transgenic pig (TG-16) pigs was stained with anti-NeuN. Layers of the pig brain cortex were indicated, showing a reduced thickness of transgenic TDP-43 pig brain cortex compared to the non-transgenic pig brain cortex. Scale bar: 200 μm