Fig. 7

Cytoplasmic mislocalization of PSF and TDP-43 in the spinal cord of transgenic pigs. a TDP-43 and PSF antibody immunocytochemistry of the spinal cord regions of non-transgenic (Control) pig and TDP-43 transgenic (TG-23) pigs. Arrows indicate the nuclei of neurons. Scale bar: 50 μm. b Immunofluorescent labeling of the spinal cord sections of the TDP-43 pig (TG-23) showing that in motor neurons (arrows) expressing transgenic TDP-43 (green), cytoplasmic distribution of PSF (red) is also evident. The nuclei were stained with Hoechst. Arrows indicate neurons in which transgenic TDP-43 is localized in both the nucleus and cytoplasm. Note that the nuclei (arrowheads) of large motor neurons were weakly labeled by Hoechst. Scale bar: 50 μm. c Flag-TDP-43 immunoprecipitation of the spinal cord sections of the TDP-43 pig revealing that PSF and NeuN were co-precipitated with cytoplasmic TDP-43 from transgenic pig tissues. C: non-transgenic. Rabbit IgG immunoprecipitation and non-transgenic pig brain tissues served as a control. d The relative levels (mean ± SE) of proteins (the ratio of IP/Input on the same blot) are presented in the right panel (n.s.: no significant difference; *p < 0.05; **p < 0.01; ***p < 0.001)