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Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: The topology of pen-2, a γ-secretase subunit, revisited: evidence for a reentrant loop and a single pass transmembrane domain

Fig. 2

The first hydrophobic region of Pen-2 is not a TMD. a. Sequence of Pen-2 HP1, SREBP TMD2 and Pen-2-SREBP chimeras. b. Cell membranes from stable cell lines expressing CT11 tagged Pen-2 (lanes 1–3) or Pen-2 TMD1 chimera (lanes 4–6) were treated with proteinase K in the absence or presence of Triton X-100 and resulting products were subject to immunoblotting with PNT2 (upper panel) or CT11 (lower panel) antibodies. c. Cell membranes from Pen-2 chimeras with substitutions of segments from the SREBP TMD2 were treated with proteinase K in the absence or presence of Triton X-100 and resulting products were subject to immunoblotting with PNT2 (upper panel) or CT11 (lower panel) antibodies. Molecular mass markers are shown on the left in kilodaltons. d. Pen-2 model proposed in Refs 17, 18 and 19. E. Model for Pen-2 TMD1. F. Revised Pen-2 model

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Molecular Neurodegeneration

ISSN: 1750-1326