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Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: Frontotemporal dementia-associated N279K tau mutant disrupts subcellular vesicle trafficking and induces cellular stress in iPSC-derived neural stem cells

Fig. 1

Generation and characterization of patient-specific iPSCs with N279K tau mutation. a Genomic DNA sequencing of the heterozygous MAPT missense mutation c.837 T > G (p.N279K) in fibroblasts from the PPND/FTDP-17 patients. b Immunostaining of pluripotency markers (Nanog, TRA-1-81 and TRA-1-60) in a control iPSC and two N279K iPSC lines. Scale bar, 50 μm. c In vitro differentiation of a control iPSC and two N279K iPSC lines into cells of all three germ layers. Cells were immunostained for AFP (endoderm), SMA (mesoderm), Tuj1/Nestin (ectoderm), and DAPI (nucleus). Scale bar, 50 μm. d-e Total mRNA levels of the reprogramming factors in a human ES cell line, a commercial iPSC line, a control iPSC line and two N279K iPSC lines relative to the values in a control fibroblast were assessed by qRT-PCR. Data are mean ± SEM from three independent experiments. f Phase-contrast images of the control iPSC line, embryonic bodies and neural rosettes. Immunostaining for the cells at different stages of neuronal differentiation; Nestin and Pax6 for NSCs, Tuj1 for mature neurons, synaptophysin for presynapses, and DAPI. Scale bar, 50 μm

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Molecular Neurodegeneration

ISSN: 1750-1326