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Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: Frontotemporal dementia-associated N279K tau mutant disrupts subcellular vesicle trafficking and induces cellular stress in iPSC-derived neural stem cells

Fig. 2

N279K neural stem cells exhibit altered tau isoform expression and enhanced cellular stress. a Immunostaining of NSC protein marker Nestin in a control and the patient-derived NSCs with N279K tau mutant. b, c Quantitative RT-PCR analysis of 3R-tau and 4R-tau mRNAs in control and N279K NSCs. Relative gene expression represents fold changes relative to that of control NSCs and normalized to β-actin expression. d Immunostaining of stress granule markers G3BP and TIA-1 in control and N279K NSCs. e-h Western blot analysis of stress granule proteins G3BP and TIA-1 in control and N279K NSCs. Quantification of Western blot densitometry of G3BP levels g and TIA-1 Levels h, normalized to GAPDH densitometry. i LDH quantification normalized to Bradford protein levels. j Caspase 3/7 activity was measured in control and N279K NSCs and the quantification was normalized to Bradford protein levels. Data are mean ± SEM from three independent experiments. *p < 0.05; **p < 0.01

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Molecular Neurodegeneration

ISSN: 1750-1326