Fig. 4

Delivery of LV-NR-R80Q-apoB reduces α-syn accumulation in MBP-α-syn tg mice. a Bright field microscopy analysis of serial longitudinal vibratome sections from the non-tg and MBP-α-syn tg mice immunostained with an antibody against total α-syn (syn-1) and analyzed for α-syn positive cells following treatment with LV-Control, or LV-NR-R80Q-apoB vector. b Computer aided image analysis for numbers of α-syn positive cells in the striatum and the corpus. c Samples that included the cortex, corpus callosum and striatum were fractioned by ultracentrifugation and analyzed by immunoblot analysis with an antibody against total α-syn (syn-1). d Densitometry analysis of the levels of α-syn immunoreactivity plotted against actin. e Laser scanning confocal microscopy of vibratome sections from the non-tg and MBP-α-syn tg immunostained with an antibody against human α-syn (syn211) and analyzed for α-syn positive cells following treatment with LV-Control, or LV-NR-R80Q-apoB vector. f Computer aided image analysis of the number of α-syn positive cells in the striatum and the corpus callosum. g Bright field microscopy analysis of serial longitudinal vibratome sections form the non-tg and MBP-α-syn tg immunostained with an antibody against phosphorylated α-syn (Ser129) and analyzed for α-syn positive cells following treatment with LV-Control, or LV-NR-R80Q-apoB vector. h Computer aided image analysis for numbers of phosphorylated α-syn positive cells in the striatum and the corpus callosum. Scale bars – top panels (low power, 40X) = 250 μm, bottom panel (high power, 400X) = 50 μm. * indicates statistical significance (p < 0.05, one way ANOVA, post hoc Dunnett’s) compared to non-tg mice LV-control. # indicates statistical significance (p < 0.05, one way ANOVA, post hoc Tukey-Kramer) compared to LV-Control treated MBP-α-syn tg mice