Fig. 2
From: The RAB39B p.G192R mutation causes X-linked dominant Parkinson’s disease
Effect of p.G192R on RAB39B expression and localization in PC12 cells. a Western blot of rat pheochromocytoma (PC12) cells transfected with a vector that was empty (mock), or contained a wild-type or mutant (p.G192R) construct. There was no difference in protein expression between wild-type and mutant RAB39B. b Immunofluorescent microscopy of nerve growth factor-differentiated PC12 cells transfected with wild-type or mutant (p.G192R) myc-tagged RAB39B. The wild-type protein is visualized throughout the cell bodies and neuritic processes, together with the vesicular marker chromogranin A, whereas the mutant protein is largely confined to cell bodies. Arrowheads indicate terminal regions of neurites. The scale bar corresponds to 10 μm