Fig. 3
From: The RAB39B p.G192R mutation causes X-linked dominant Parkinson’s disease
Effect of p.G192R on RAB39B expression and localization in SK-N-BE(2)C cells. a Western blot of human neuroblastoma (SK-N-BE(2)C) cells transfected with a vector that was empty (mock), or contained a wild-type or mutant (p.G192R) construct. There was no significant difference in protein expression between wild-type and mutant RAB39B (Student's t-test, p > 0.05. b Immunofluorescent microscopy of retinoic acid-differentiated SK-N-BE(2)C cells transfected with wild-type or mutant (p.G192R) GFP-tagged RAB39B. Wild-type protein was seen within the cytoplasm and at the plasma membrane where it co-localized with epidermal growth factor receptor (EGFR; blue arrows). Although mutant RAB39B protein was also apparent in the cytoplasm, it less often co-localized with EGFR (white arrows). The scale bar corresponds to 10 μm. c Immunoblot analysis of fractionated protein extracts performed on SK-N-BE(2)C cells transfected with a vector that was empty (mock), or contained GFP-tagged wild-type or mutant RAB39B. Double asterisks indicate a significant difference (p < 0.01) by Student’s t-test (n = 3 replicates)