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Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: Region-specific dendritic simplification induced by Aβ, mediated by tau via dysregulation of microtubule dynamics: a mechanistic distinct event from other neurodegenerative processes

Fig. 1

Analysis of dendritic simplification in an ex vivo model of Alzheimer’s disease. a Outline of the experimental approach. 400 μm-thick hippocampal sections from APPSDL transgenic or non-transgenic mice were cultured using the membrane interface technique. Sindbis virus infection to express EGFP-tagged constructs was performed at day 12 in vitro (DIV). Slices were fixed three days later as indicated on the time line. b Fluorescence micrograph of a whole slice showing infected and EGFP-expressing neurons in DG, CA1 and CA3 regions. Scale bar, 150 μm. c High resolution fluorescence micrograph of a CA1 pyramidal neuron after semi-automated stitching (left) and 3D reconstruction (right). The apical part of the dendritic tree is indicated in red, the basal part in blue and the axon in turquoise. Scale bar, 50 μm. d 3D reconstructions of representative CA1, CA3 and DG neurons from APPSDL transgenic and non-transgenic (B6) mice after expression of EGFP-htau

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