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Table 2 Effect of drug treatment on dendritic tau levels. Dendritic tau levels in 2nd or 3rd order of CA1 neurons expressing the respective construct were determined as described in “Methods”. For every neuron, 3–4 measurements were performed and averaged. Statistical evaluation was performed using two-tailed, unpaired Student’s t test for untreated cultures, and one way ANOVA with post hoc Fisher’s LSD test for multiple comparisons of drug treatments or different tau constructs. Note that alpha levels of ANOVA did not reach significance (p≤0.05) for any condition

From: Region-specific dendritic simplification induced by Aβ, mediated by tau via dysregulation of microtubule dynamics: a mechanistic distinct event from other neurodegenerative processes

Construct

Condition

Region

Genotype

Mice (n)

Cells (n)

Mean ± s.e.m (based on cell number) of normalized intensity values

Student’s t test

  

p values (B6 vs. APP)

EGFP-htau

untreated

CA1

B6

3

5

1.061±0.099

p = 0.6210

EGFP-htau

untreated

CA1

APPSDL

5

5

1.111±0.120

Construct

Condition

Region

Genotype

Mice (n)

Cells (n)

Mean ± s.e.m (based on cell number) of normalized intensity values

One way ANOVA (together with EGFP-htau untreated, both genotypes)

p values (B6 vs. APP)

p values (vs. untreated same genotype)

EGFP-htau

DAPT

CA1

B6

3

6

0.827±0.066

F (3,17) = 2.183 ; p = 0.1275

p = 0.0273

p = 0.2007

EGFP-htau

DAPT

CA1

APPSDL

4

5

1.197±0.154

p = 0.5981

EGFP-htau

EpoD

CA1

B6

4

5

1.119±0.138

F (3,16) = 0.2108 ; p = 0.8874

p = 0.8533

p = 0.5763

EGFP-htau

EpoD

CA1

APPSDL

5

5

1.148±0.066

p = 0.8135

EGFP-htau

CPP

CA1

B6

5

5

0.945±0.176

F (3,16) = 1.334 ; p = 0.2984

p = 0.3752

p = 0.6129

EGFP-htau

CPP

CA1

APPSDL

4

5

0.793±0.019

p = 0.0745

EGFP-Ala htau

untreated

CA1

B6

5

5

1.083±0.135

F (3,16) = 0.6240 ; p = 0.6098

p = 0.2952

p = 0.7390

EGFP-Ala htau

untreated

CA1

APPSDL

5

5

0.917±0.068

p = 0.2240

EGFP-PHP htau

untreated

CA1

B6

3

5

1.174±0.064

F (3,16) = 0.5229 ; p = 0.6727

p = 0.9881

p = 0.2937

EGFP-PHP htau

untreated

CA1

APPSDL

3

5

1.172±0.080

p = 0.6495