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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: SOD1 protein aggregates stimulate macropinocytosis in neurons to facilitate their propagation

Fig. 3

Endosome rupture by SOD1 aggregates. NSC-34 cells were incubated with either PBS (digitonin and Triton X-100 treatments), non-aggregated wt or G93A SOD1 or aggregated G93A SOD1 for 2 h before permeabilizatin (digitonin for the SOD1 treatments), fixation, immunostaining of galectin-3 and the mean fluorescence per cell quantified. A minimum of 150 cells were counted per treatment across a minimum of 5 fields of view per treatment and the mean galectin-3 fluorescence per cell calculated. Results shown are means ± SE, ** p < 0.01, *** p <0.001. Confocal microscopy of the immunostaining of galectin-3 after treatment with PBS and the SOD1 preparations, counterstained with RedDot

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